human integrin αvβ5 Search Results


human integrin alpha v beta 5 antibody  (Bio-Techne corporation)
99
Bio-Techne corporation human integrin alpha v beta 5 antibody
Human Integrin Alpha V Beta 5 Antibody, supplied by Bio-Techne corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human integrin alpha v beta 5 antibody/product/Bio-Techne corporation
Average 99 stars, based on 1 article reviews
human integrin alpha v beta 5 antibody - by Bioz Stars, 2026-03
99/100 stars
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a375s.2 human melanoma cell line  (Centocor Inc)
90
Centocor Inc a375s.2 human melanoma cell line
A375s.2 Human Melanoma Cell Line, supplied by Centocor Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/a375s.2 human melanoma cell line/product/Centocor Inc
Average 90 stars, based on 1 article reviews
a375s.2 human melanoma cell line - by Bioz Stars, 2026-03
90/100 stars
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soluble human integrins α3β1, αvβ3, αvβ5 α5β1  (Upstate Biotechnology Inc)
90
Upstate Biotechnology Inc soluble human integrins α3β1, αvβ3, αvβ5 α5β1
A. KSHV internalization in the presence of heparin and mannan. 1×106 THP-1 cells mock treated or pre-incubated with the indicated concentrations of mannan at 4°C were infected with 10 KSHV DNA copies per cell or heparin pretreated KSHV (100ug/ml). At different time points, uninternalized virus was removed by washing and trypsinization. Infected and mock infected cells were washed and internalized viral copy numbers were determined by real-time DNA PCR as described in the figure 1C/D legend. The data are represented as the percent inhibition of KSHV DNA internalized in comparison with cells incubated with virus alone. Each reaction was done in duplicate and each bar represents the mean ± SD of the results of three experiments. B. KSHV internalization in THP1 cells upon blocking DC-SIGN. THP1 cells incubated with mouse IgG or mouse anti-DC-SIGN mAb at 4° C for 1 h. The washed cells were incubated with KSHV (10 genome copies/cell) at 4° C for 1 h followed by at 37° C for 1 h. Uninternalized virus was removed by washing and trypsinization, internalized viral copy numbers were determined by real-time DNA PCR as described in the figure 1C/D legend. The data are represented as the percent inhibition of KSHV DNA internalization in comparison with IgG control. C. KSHV gene expression in the presence of soluble integrins. 1×106 THP-1 cells were infected with 30 KSHV DNA copies per cell of untreated virus or with virus pretreated with 10μg/ml of soluble α3β1, αvβ3, αvβ5, <t>α5β1</t> and α4β7 integrins for 1 h at 37° C. At 2 h p.i. uninternalized virus was removed by washing and trypsinization and the infected cells were further incubated. At 24 h p.i., the cells were washed, RNA was isolated, ORF73 gene expression was examined as described under the figure 2B legend. ORF73 gene expression was represented as percent inhibition compared to control untreated KSHV infection.
Soluble Human Integrins α3β1, αvβ3, αvβ5 α5β1, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/soluble human integrins α3β1, αvβ3, αvβ5 α5β1/product/Upstate Biotechnology Inc
Average 90 stars, based on 1 article reviews
soluble human integrins α3β1, αvβ3, αvβ5 α5β1 - by Bioz Stars, 2026-03
90/100 stars
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recombinant human integrin αvβ5 it5-h52w5  (ACROBiosystems)
90
ACROBiosystems recombinant human integrin αvβ5 it5-h52w5
A. KSHV internalization in the presence of heparin and mannan. 1×106 THP-1 cells mock treated or pre-incubated with the indicated concentrations of mannan at 4°C were infected with 10 KSHV DNA copies per cell or heparin pretreated KSHV (100ug/ml). At different time points, uninternalized virus was removed by washing and trypsinization. Infected and mock infected cells were washed and internalized viral copy numbers were determined by real-time DNA PCR as described in the figure 1C/D legend. The data are represented as the percent inhibition of KSHV DNA internalized in comparison with cells incubated with virus alone. Each reaction was done in duplicate and each bar represents the mean ± SD of the results of three experiments. B. KSHV internalization in THP1 cells upon blocking DC-SIGN. THP1 cells incubated with mouse IgG or mouse anti-DC-SIGN mAb at 4° C for 1 h. The washed cells were incubated with KSHV (10 genome copies/cell) at 4° C for 1 h followed by at 37° C for 1 h. Uninternalized virus was removed by washing and trypsinization, internalized viral copy numbers were determined by real-time DNA PCR as described in the figure 1C/D legend. The data are represented as the percent inhibition of KSHV DNA internalization in comparison with IgG control. C. KSHV gene expression in the presence of soluble integrins. 1×106 THP-1 cells were infected with 30 KSHV DNA copies per cell of untreated virus or with virus pretreated with 10μg/ml of soluble α3β1, αvβ3, αvβ5, <t>α5β1</t> and α4β7 integrins for 1 h at 37° C. At 2 h p.i. uninternalized virus was removed by washing and trypsinization and the infected cells were further incubated. At 24 h p.i., the cells were washed, RNA was isolated, ORF73 gene expression was examined as described under the figure 2B legend. ORF73 gene expression was represented as percent inhibition compared to control untreated KSHV infection.
Recombinant Human Integrin αvβ5 It5 H52w5, supplied by ACROBiosystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant human integrin αvβ5 it5-h52w5/product/ACROBiosystems
Average 90 stars, based on 1 article reviews
recombinant human integrin αvβ5 it5-h52w5 - by Bioz Stars, 2026-03
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anti-human αvβ5 integrin mab  (Merck & Co)
90
Merck & Co anti-human αvβ5 integrin mab
A. KSHV internalization in the presence of heparin and mannan. 1×106 THP-1 cells mock treated or pre-incubated with the indicated concentrations of mannan at 4°C were infected with 10 KSHV DNA copies per cell or heparin pretreated KSHV (100ug/ml). At different time points, uninternalized virus was removed by washing and trypsinization. Infected and mock infected cells were washed and internalized viral copy numbers were determined by real-time DNA PCR as described in the figure 1C/D legend. The data are represented as the percent inhibition of KSHV DNA internalized in comparison with cells incubated with virus alone. Each reaction was done in duplicate and each bar represents the mean ± SD of the results of three experiments. B. KSHV internalization in THP1 cells upon blocking DC-SIGN. THP1 cells incubated with mouse IgG or mouse anti-DC-SIGN mAb at 4° C for 1 h. The washed cells were incubated with KSHV (10 genome copies/cell) at 4° C for 1 h followed by at 37° C for 1 h. Uninternalized virus was removed by washing and trypsinization, internalized viral copy numbers were determined by real-time DNA PCR as described in the figure 1C/D legend. The data are represented as the percent inhibition of KSHV DNA internalization in comparison with IgG control. C. KSHV gene expression in the presence of soluble integrins. 1×106 THP-1 cells were infected with 30 KSHV DNA copies per cell of untreated virus or with virus pretreated with 10μg/ml of soluble α3β1, αvβ3, αvβ5, <t>α5β1</t> and α4β7 integrins for 1 h at 37° C. At 2 h p.i. uninternalized virus was removed by washing and trypsinization and the infected cells were further incubated. At 24 h p.i., the cells were washed, RNA was isolated, ORF73 gene expression was examined as described under the figure 2B legend. ORF73 gene expression was represented as percent inhibition compared to control untreated KSHV infection.
Anti Human αvβ5 Integrin Mab, supplied by Merck & Co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-human αvβ5 integrin mab/product/Merck & Co
Average 90 stars, based on 1 article reviews
anti-human αvβ5 integrin mab - by Bioz Stars, 2026-03
90/100 stars
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Image Search Results


A. KSHV internalization in the presence of heparin and mannan. 1×106 THP-1 cells mock treated or pre-incubated with the indicated concentrations of mannan at 4°C were infected with 10 KSHV DNA copies per cell or heparin pretreated KSHV (100ug/ml). At different time points, uninternalized virus was removed by washing and trypsinization. Infected and mock infected cells were washed and internalized viral copy numbers were determined by real-time DNA PCR as described in the figure 1C/D legend. The data are represented as the percent inhibition of KSHV DNA internalized in comparison with cells incubated with virus alone. Each reaction was done in duplicate and each bar represents the mean ± SD of the results of three experiments. B. KSHV internalization in THP1 cells upon blocking DC-SIGN. THP1 cells incubated with mouse IgG or mouse anti-DC-SIGN mAb at 4° C for 1 h. The washed cells were incubated with KSHV (10 genome copies/cell) at 4° C for 1 h followed by at 37° C for 1 h. Uninternalized virus was removed by washing and trypsinization, internalized viral copy numbers were determined by real-time DNA PCR as described in the figure 1C/D legend. The data are represented as the percent inhibition of KSHV DNA internalization in comparison with IgG control. C. KSHV gene expression in the presence of soluble integrins. 1×106 THP-1 cells were infected with 30 KSHV DNA copies per cell of untreated virus or with virus pretreated with 10μg/ml of soluble α3β1, αvβ3, αvβ5, α5β1 and α4β7 integrins for 1 h at 37° C. At 2 h p.i. uninternalized virus was removed by washing and trypsinization and the infected cells were further incubated. At 24 h p.i., the cells were washed, RNA was isolated, ORF73 gene expression was examined as described under the figure 2B legend. ORF73 gene expression was represented as percent inhibition compared to control untreated KSHV infection.

Journal:

Article Title: Characterization of Entry and Infection of Monocytic THP-1 cells by Kaposi's Sarcoma Associated Herpesvirus (KSHV): Role of Heparan Sulfate, DC-SIGN, Integrins and Signaling

doi: 10.1016/j.virol.2010.07.012

Figure Lengend Snippet: A. KSHV internalization in the presence of heparin and mannan. 1×106 THP-1 cells mock treated or pre-incubated with the indicated concentrations of mannan at 4°C were infected with 10 KSHV DNA copies per cell or heparin pretreated KSHV (100ug/ml). At different time points, uninternalized virus was removed by washing and trypsinization. Infected and mock infected cells were washed and internalized viral copy numbers were determined by real-time DNA PCR as described in the figure 1C/D legend. The data are represented as the percent inhibition of KSHV DNA internalized in comparison with cells incubated with virus alone. Each reaction was done in duplicate and each bar represents the mean ± SD of the results of three experiments. B. KSHV internalization in THP1 cells upon blocking DC-SIGN. THP1 cells incubated with mouse IgG or mouse anti-DC-SIGN mAb at 4° C for 1 h. The washed cells were incubated with KSHV (10 genome copies/cell) at 4° C for 1 h followed by at 37° C for 1 h. Uninternalized virus was removed by washing and trypsinization, internalized viral copy numbers were determined by real-time DNA PCR as described in the figure 1C/D legend. The data are represented as the percent inhibition of KSHV DNA internalization in comparison with IgG control. C. KSHV gene expression in the presence of soluble integrins. 1×106 THP-1 cells were infected with 30 KSHV DNA copies per cell of untreated virus or with virus pretreated with 10μg/ml of soluble α3β1, αvβ3, αvβ5, α5β1 and α4β7 integrins for 1 h at 37° C. At 2 h p.i. uninternalized virus was removed by washing and trypsinization and the infected cells were further incubated. At 24 h p.i., the cells were washed, RNA was isolated, ORF73 gene expression was examined as described under the figure 2B legend. ORF73 gene expression was represented as percent inhibition compared to control untreated KSHV infection.

Article Snippet: Rabbit anti-integrin α3 and β1 and soluble human integrins α3β1, αvβ3, αvβ5 and α5β1 were from Upstate Biotechnology, Lake Placid, N.Y. Recombinant human integrin α4β7 was from R&D systems, Minneapolis, MN.

Techniques: Incubation, Infection, Inhibition, Blocking Assay, Expressing, Isolation

THP-1 cells were infected with 10 KSHV DNA copies per cell for 5 and 10 min. The cells were fixed in 2 % paraformaldehyde for 10 min, permeabilized with 0.2% Triton X-100 for 5 min and blocked. Infected and uninfected cells were stained with anti-KSHV gB and anti-α3β1/αvβ3/αvβ5/α5β1/αvβ6 integrin antibodies and visualized by confocal microscopy. KSHV-gB and integrins were visualized by incubation with Alexa-488 secondary antibody (green) and Alexa-594 secondary antibody (red), respectively. The arrows indicate the areas of KSHV co-localization with integrins. A. KSHV+ α3β1; B. KSHV+ αvβ3; C. KSHV+ αvβ5; D. KSHV+ α5β1; E. KSHV+ αvβ6.

Journal:

Article Title: Characterization of Entry and Infection of Monocytic THP-1 cells by Kaposi's Sarcoma Associated Herpesvirus (KSHV): Role of Heparan Sulfate, DC-SIGN, Integrins and Signaling

doi: 10.1016/j.virol.2010.07.012

Figure Lengend Snippet: THP-1 cells were infected with 10 KSHV DNA copies per cell for 5 and 10 min. The cells were fixed in 2 % paraformaldehyde for 10 min, permeabilized with 0.2% Triton X-100 for 5 min and blocked. Infected and uninfected cells were stained with anti-KSHV gB and anti-α3β1/αvβ3/αvβ5/α5β1/αvβ6 integrin antibodies and visualized by confocal microscopy. KSHV-gB and integrins were visualized by incubation with Alexa-488 secondary antibody (green) and Alexa-594 secondary antibody (red), respectively. The arrows indicate the areas of KSHV co-localization with integrins. A. KSHV+ α3β1; B. KSHV+ αvβ3; C. KSHV+ αvβ5; D. KSHV+ α5β1; E. KSHV+ αvβ6.

Article Snippet: Rabbit anti-integrin α3 and β1 and soluble human integrins α3β1, αvβ3, αvβ5 and α5β1 were from Upstate Biotechnology, Lake Placid, N.Y. Recombinant human integrin α4β7 was from R&D systems, Minneapolis, MN.

Techniques: Infection, Staining, Confocal Microscopy, Incubation